Single Particle Analysis Simulations

Single Particle Analysis Schematic

• Reconstructs 3D volumes of homogeneous samples from a large number of 2D EM randomly-oriented micrographs
• The main steps involve:
  • Obtaining cryo-EM micrographs
  • Automated "particle" identification
  • Class averaging
  • Orientation estimation
  • Tomographic reconstruction

STEM Simulations

T20S Proteasome

• We use a solved structure, namely the T20S proteasome

Projected Potential

• We use a solved structure, namely the T20S proteasome
• We simulate a large FOV using 16x16 tiled proteasome volumes, each randomly rotated about its center
  • 300 kV, 2.5μm defocus, 3 mrad convergence angle

Noisy Diffraction Patterns

• We use a solved structure, namely the T20S proteasome
• We simulate a large FOV using 16x16 tiled proteasome volumes, each randomly rotated about its center
  • 300 kV, 2.5μm defocus, 3 mrad convergence angle
• We use a finite dose of 30 e/Å$^2$

Single Slice Reconstruction

• We start by reconstructing the entire FOV using single-slice ptychography